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    10x Protocols: Chromium Single Cell/Nuclei Gene Expression Flex Fixation for sc/sn -- University of Minnesota TMCs (CG000478 Rev A)

    IOx Genomics, Laura Niedernhofer

    Published: 2023-06-26 DOI: 10.17504/protocols.io.ewov1opq2lr2/v1
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    Abstract

    DOIs for dissociation protocols and 10x Genomics fixation for Chromium Single Cell Expression flex protocols.

    Protocol ID# (CG) and Revision letter provided here :

    10x CG000478,  **Revision A** -- Protocol for fixation of single cells and nuclei prior to applying 10x Genomics Chromium flex protocols.

    Note: These protocols may not be the current version offered by the company but were used to produce the specific datasets connected to them. Please review the company support websites for the most recent versions of the protocols prior to starting your experiment.

    Steps

    1.

    http://www.10xgenomics.com/products/single-cell-gene-expression-flex

    2.

    10x Protocol CG000478, Rev A (Fixation):

    10x Fixation of Cells and Nuclei (CG000478, Rev A).pdf

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