Comparative analysis of assays to measure CAR T-cell-mediated cytotoxicity

Stefan Kiesgen, John C. Messinger, Navin K. Chintala, Zachary Tano, Prasad S. Adusumilli

Published: 2021-02-14 DOI: 10.1038/s41596-020-00467-0

CAR T-cell

cytotoxicity assay

immune effector cells

antitumor efficacy

FDA directives

AI 解读

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References

The antitumor efficacy of genetically engineered ‘living drugs’, including chimeric antigen receptor and T-cell receptor Tcells, is influenced by their activation, proliferation, inhibition, and exhaustion. A sensitive and reproducible cy
Main
51Cr assay
BLI luciferase assay
Impedance-based assay
Flow assay
Alternative assays
Advanced invitro antitumor efficacy assays
Potency assays for clinical cell therapy products
Assessment of cell therapies beyond cytotoxicity
Discussion
References
ACKNOWLEDGMENTS

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Comparative analysis of assays to measure CAR T-cell-mediated cytotoxicity

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