Designing and executing prime editing experiments in mammalian cells

David R. Liu, Jordan L. Doman, Alexander A. Sousa, Peyton B. Randolph, Peter J. Chen

Published: 2022-08-07 DOI: 10.1038/s41596-022-00724-4

Gene editing

Prime editing

Mammalian cells

PegRNA design

PE systems

AI 解读

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Prime editing (PE) is a precision gene editing technology that enables the programmable installation of substitutions, insertions and deletions in cells and animals without requiring double-strand DNA breaks (DSBs). The mechanism of PE makes it less depen
Introduction
Procedure
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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Designing and executing prime editing experiments in mammalian cells

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Reporting Summary

Supplementary Table 1

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