Fresh Frozen / OCT Tissue RNA Quality Evaluation -- University of Minnesota TMCs (ThermoFisher Scientific, Invitrogen - Purelink)
Laura J Niedernhofer, David A Bernlohr, ThermoFisher Scientific
Published: 2023-08-10 DOI: 10.17504/protocols.io.dm6gpjpz8gzp/v1
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Abstract
RNA quality evaluation of OCT (Optimal cutting temperature) or fresh frozen blocks/samples.
Completed prior to going through -omics and spatial technologies, sc/snRNAseq if applicable.
Steps
Purelink RNA Mini Kit Manual
1.
purelink_rna_mini_kit_man -- Reduced to pages of interest.pdf
2.
Product Information
Catalog Number: 12183018A
http://www.thermofisher.com/order/catalog/product/12183018A
or
Catalog Number: 12183020
http://www.thermofisher.com/order/catalog/product/12183020
3.
Complete:
Nanodrop for RNA quantification
RIN score for (Eukaryotic total) RNA qualification
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