Overall protocol for top-down LC-MS/MS of human lung tissue
Jeannie Camarillo, Bryon Drown, Neil Kelleher
Published: 2022-03-02 DOI: 10.17504/protocols.io.b5sbq6an
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Abstract
Overview description of the process for acquiring top-down LC-MS/MS data on human lung tissue
Steps
Tissue Collection
1.
Lung tissue was provided by University of Rochester Medical Center. Tissue was collected and prepared according to the following:
615.1 URMC HTC Non-Inflated Fresh-Frozen Embedded Lung and Associated Tissue
Sample Preparation
2.
Perform LC based Proteomics on adjacent tissue sections:
Tissue Homogenization and GELFrEE for Top-Down Proteomics
Data Acquisition
3.
Untargeted Top-down Proteomics by LC-MS/MS on Eclipse
4.
Top-down proteomics data analysis on the NRTDP TDPortal
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