Protocol 1 Keira Smith Published: 2021-08-24 DOI: 10.17504/protocols.io.bwgfpbtn mykeyword1 mykeyword2 AI 解读 浏览 1312 Abstract Main Steps1. Step 1 推荐阅读 Nature Protocols Protocols IO Current Protocols Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail Profiling native pulmonary basement membrane stiffness using atomic force microscopy Genetic circuit design automation with Cello 2.0 Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2) A practical guide to scanning light-field microscopy with digital adaptive optics Effective high-throughput isolation of fully human antibodies targeting infectious pathogens Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments MOF-derived nanoporous carbons with diverse tunable nanoarchitectures Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease 查看全部 Intrahepatic implantation of tumor cells Intrahepatic tumor implantation Differential transcript usage analysis Small volume fungal genomic DNA extraction protocol for Illumina genome BIDMC TMC - low input single nuclei sequencing isolation (NNLB) Preparation of 1M calcium chloride solution (CaCl2) ngm worm c.elegans c elegans elegans caenorhabditis media medium calcium chloride Gradient PCR with DMSO gDNA RNA Clean Up Protocol Processamento de actígrafos pré-coleta - ActTrust actigrafia sono cronobiologia condor instruments Multiplex PCR Assay for Clade-typing Salmonella Enteritidis Non-typhoidal Salmonella Real-time PCR Phylogeny Molecular surveillance Generating a low-copy overexpression cell line using PhiC31 integration phiC31 overexpression attb 查看全部 Optimized and Scalable Precoating-Free Reprogramming of Human Peripheral Blood Mononuclear Cells into iPSCs coating extracellular matrix induced pluripotent stem cells laminin-511 reprogramming PBMCs scaling down Methods to Detect Small Molecule Inhibition of RING E3 Ligase Activity alpha screen RING E3 ligase small molecules TRAF6 Ubc13 ubiquitination Reprogramming Urine-Derived Cells Using Commercially Available Self-Replicative RNA and a Single Electroporation induced pluripotent stem cell reprogramming self-replicative RNA urine Fluorescent Protein Production, Purification, and Coupling to Microspheres fluorescent proteins full-spectrum cytometry microspheres protein expression protein purification RNA Extraction from Plant Tissue with Homemade Acid Guanidinium Thiocyanate Phenol Chloroform (AGPC) AGPC home-made buffers molecular biology protocol multiple plant species and tissues nucleic acids RNA isolation Production and Thermal Exchange of Conditional Peptide-MHC I Multimers CD8+ T cell staining MHC class I multimer peptide thermal exchange A Step-by-Step Guide for the Production of Recombinant Fluorescent TAT-HA-Tagged Proteins and their Transduction into Mammalian Cells DNA molecular assembly fluorescent protein fusion protein Ni-NTA-based protein purification protein transduction recombinant protein production tagged protein Sperm Chromatin Structure Assay (SCSA®) for Fertility Assessment DNA integrity flow cytometry male fertility pregnancy outcomes SCSA sperm chromatin structure assay ntLink: A Toolkit for De Novo Genome Assembly Scaffolding and Mapping Using Long Reads de novo genome assembly long reads minimizer mapping scaffolding ntLink An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat Agrobacterium CRISPR genome editing immature embryo wheat transformation 查看全部
