Protocol for Mouse IP Anesthesia (Ketamine-Xylazine)
Mohsen Khosravi-Maharlooei, Markus Holzl, Austin Chen, Megan Sykes, Haowei Li, Nichole Danzl
Abstract
This protocol details the constitution of our anesthesia cocktail that we use for sedating our mice when performing surgeries. The surgeries we typically perform are thymectomies and implantation of material under the kidney capsule (through open abdomen or minimally invasive approaches).
Steps
For Intraperitoneal (IP) Approach:
Use 10 uL/gram of anesthesia
Comments:
1. It takes time until the mice fall asleep
2. Very often, mice do not fall asleep but move around like zombies. This requires another small dose of IP ketamine-xylazine injection or a small dose of IV injection
a. When doing IP injection again, we have injected IP with a volume of about 1/3 of original volume
b. For IV approach after IP, it takes very little anesthesia for them to fall asleep. We usually use around 10-20 uL. Inject this slowly into the mouse. The mouse should fall asleep rapidly. It is very easy to overdose with IV injection so be careful.
