Revealing nascent RNA processing dynamics with nano-COP

Heather L. Drexler, Karine Choquet, Hope E. Merens, Paul S. Tang, Jared T. Simpson, L. Stirling Churchman

Published: 2021-01-28 DOI: 10.1038/s41596-020-00469-y

nanopore sequencing

nano-COP

RNA processing

introns splicing

nascent RNA

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During maturation, eukaryotic precursor RNAs undergo processing events including intron splicing, 3′-end cleavage, and polyadenylation. Here we describe nanopore analysis of co-transcriptional processing (nano-COP), a method for probing the timing
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS

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Revealing nascent RNA processing dynamics with nano-COP

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