Simultaneously measuring the methylation of parent and daughter strands of replicated DNA at the single-molecule level by Hammer-seq

Xuan Ming, Bing Zhu, Zhuqiang Zhang

Published: 2021-03-07 DOI: 10.1038/s41596-020-00488-9

kinetics

Hammer-seq

DNA methylation

single-molecule level

replicated DNA

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The stable maintenance of DNA methylation patterns during mitotic cell division is crucial for cell identity. Precisely determining the maintenance kinetics and dissecting the exact contributions of relevant regulators requires a method to accurately meas
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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ACKNOWLEDGMENTS

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Simultaneously measuring the methylation of parent and daughter strands of replicated DNA at the single-molecule level by Hammer-seq

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